Accessibility navigation


A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals

Crabbe, M. J. C. (2003) A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals. Journal of Biochemical and Biophysical Methods, 57 (2). pp. 171-176. ISSN 0165-022X

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1016/s0165-022x(03)00051-4

Abstract/Summary

We have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA(R) card (19 mug-43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large Subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 mug total DNA (S. siderea coral DNA) and 9 mug total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular Study of a far wider range and variety of coral sites than have been studied to date. (C) 2003 Elsevier Science B.V. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10714
Uncontrolled Keywords:FTA cards, DNA, rRNA, zooxanthellae, cnidaria, GENUS MADRACIS, SE SULAWESI, REEF, RECRUITMENT, INDONESIA, SAMPLES, SITES, PAPER, CARDS, PARK

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation