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Induction of aquaporin 1 but not aquaporin 4 messenger RNA in rat primary brain microvessel endothelial cells in culture

Dolman, D., Drndarski, S., Abbott, N. J. and Rattray, M. (2005) Induction of aquaporin 1 but not aquaporin 4 messenger RNA in rat primary brain microvessel endothelial cells in culture. Journal of Neurochemistry, 93 (4). pp. 825-33. ISSN 1471-4159

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To link to this article DOI: 10.1111/j.1471-4159.2005.03111.x

Abstract/Summary

Aquaporins (AQPs) are a family of proteins that mediate water transport across cells, but the extent to which they are involved in water transport across endothelial cells of the blood-brain barrier is not clear. Expression of AQP1 and AQP4 in rat brain microvessel endothelial cells was investigated in order to determine whether these isoforms were present and, in particular, to examine the hypothesis that brain endothelial expression of AQPs is dynamic and regulated by astrocytic influences. Reverse-transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry showed that AQP1 mRNA and protein are present at very low levels in primary rat brain microvessel endothelial cells, and are up-regulated in passaged cells. Upon passage, endothelial cell expression of mdr1a mRNA is decreased, indicating loss of blood-brain barrier phenotype. In passage 4 endothelial cells, AQP1 mRNA levels are reduced by coculture above rat astrocytes, demonstrating that astrocytic influences are important in maintaining the low levels of AQP1 characteristic of the blood-brain barrier endothelium. Reverse-transcriptase-PCR revealed very low levels of AQP1 mRNA present in the RBE4 rat brain microvessel endothelial cell line, with no expression detected in primary cultures of rat astrocytes or in the C6 rat glioma cell line. In contrast, AQP4 mRNA is strongly expressed in astrocytes, but no expression is found in primary or passaged brain microvessel endothelial cells, or in RBE4 or C6 cells. Our results support the concept that expression of AQP1, which is seen in many non-brain endothelia, is suppressed in the specialized endothelium of the blood-brain barrier.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
ID Code:15728
Uncontrolled Keywords:Actins/genetics/metabolism Animals Antigens, CD11b/genetics/metabolism Aquaporin 1 Aquaporin 4 Aquaporins/genetics/*metabolism Blotting, Northern/methods Brain/*cytology Cells, Cultured Endothelial Cells/*metabolism Endothelium, Vascular/*cytology Gene Expression Regulation/*physiology Glial Fibrillary Acidic Protein/genetics/metabolism Immunohistochemistry/methods Male P-Glycoprotein/genetics/metabolism RNA, Messenger/metabolism Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction/methods Time Factors
Publisher:International Society for Neurochemistry

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