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Isolation and characterisation of EfeM, a periplasmic component of the putative EfeUOBM iron transporter of Pseudomonas syringae pv. syringae

Rajasekaran, M. B., Mitchell, S. A., Gibson, T. M., Hussain, R., Siligardi, G., Andrews, S. C. and Watson, K. A. (2010) Isolation and characterisation of EfeM, a periplasmic component of the putative EfeUOBM iron transporter of Pseudomonas syringae pv. syringae. Biochemical and Biophysical Research Communications, 398 (3). pp. 366-371. ISSN 0006-291X

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To link to this article DOI: 10.1016/j.bbrc.2010.06.072

Abstract/Summary

The EfeM protein is a component of the putative EfeUOBM iron-transporter of Pseudomonas syringae pathovar syringae and is thought to act as a periplasmic, ferrous-iron binding protein. It contains a signal peptide of 34 amino acid residues and a C-terminal 'Peptidase_M75' domain of 251 residues. The C-terminal domain contains a highly conserved 'HXXE' motif thought to act as part of a divalent cation-binding site. In this work, the gene (efeM or 'Psyr_3370') encoding EfeM was cloned and over-expressed in Escherichia coli, and the mature protein was purified from the periplasm. Mass spectrometry confirmed the identity of the protein (M(W) 27,772Da). Circular dichroism spectroscopy of EfeM indicated a mainly alpha-helical structure, consistent with bioinformatic predictions. Purified EfeM was crystallised by hanging-drop vapor diffusion to give needle-shaped crystals that diffracted to a resolution of 1.6A. This is the first molecular study of a peptidase M75 domain with a presumed iron transport role.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
ID Code:17905
Uncontrolled Keywords: Iron transport; Peptidase_M75; SRCD; Mass spectrometry; Crystallisation; EfeUOB; Psyr_3370
Publisher:Elsevier

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