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Manipulations of germ-cell populations in the gonad of the fowl

Bresler, M., Behnam, J., Luke, G. and Simkiss, K. (1994) Manipulations of germ-cell populations in the gonad of the fowl. British Poultry Science, 35 (2). pp. 241-247. ISSN 1466-1799

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To link to this article DOI: 10.1080/00071669408417688

Abstract/Summary

1. Embryos of the domestic fowl have been partially sterilised by injecting the drug busulphan into 24-h incubated eggs. 2. Some of these embryos were injected with primordial germ cells (PGCs) after 55 h of incubation to attempt to repopulate the gonads. 3. Primordial germ cells transfected with a defective retrovirus containing the reporter gene lac Z were shown to settle in these sterilised gonads. 4. Quantitative histology of 6-d embryos showed that busulphan produced 75% sterilisation but that PGCs could repopulate these gonads. 5. The technique of producing such germ line chimaeras is of value in studying cell kinetics, gonad differentiation and the production of transgenics.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:23821
Uncontrolled Keywords:Animals Busulfan/*administration & dosage/toxicity Chick Embryo/cytology/*drug effects Genes, Bacterial Germ Cells/cytology/drug effects *Sterilization, Reproductive/methods Transfection beta-Galactosidase/biosynthesis
Additional Information:Bresler, M Behnam, J Luke, G Simkiss, K Research Support, Non-U.S. Gov't Scotland British poultry science Br Poult Sci. 1994 May;35(2):241-7.
Publisher:Taylor & Francis

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