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Female chromosomes in cockerel ejaculates

Simkiss, K., Luke, G. and Behnam, J. (1996) Female chromosomes in cockerel ejaculates. Proceedings of the Royal Society B-Biological Science, 263 (1374). pp. 1245-1249. ISSN 0962-8452

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To link to this article DOI: 10.1098/rspb.1996.0183

Abstract/Summary

We describe a polymerase chain reaction which amplifies part of the Eco RI repeat unit of the fowl W chromosome. The resulting 447 bp fragment enables DNA from female birds to be identified. The composition of this DNA is confirmed by a nested polymerase chain reaction which specifically amplifies a known internal 263 bp region in this fragment. Using this technique it is possible to follow the fate of female cells in male germline chimaeras. The polymerase chain reaction fragment can be traced in cells of the embryonic and hatchling gonad and in adult sperm implying that cells containing the W chromosome are capable of being processed through the avian testis.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:23838
Uncontrolled Keywords:Animals Birds Female Male Molecular Sequence Data Polymerase Chain Reaction Sex Chromosomes/*genetics *Sex Determination Analysis
Additional Information:Simkiss, K Luke, G Behnam, J Research Support, Non-U.S. Gov't England Proceedings. Biological sciences / The Royal Society Proc Biol Sci. 1996 Sep 22;263(1374):1245-9.
Publisher:Royal Society Publishing

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