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Classification of cortical microcircuits based on micro-electrode-array data from slices of rat barrel cortex

Bakker, R., Schubert, D., Levels, K., Bezgin, G., Bojak, I. and Kötter, R. (2009) Classification of cortical microcircuits based on micro-electrode-array data from slices of rat barrel cortex. Neural Networks, 22 (8). pp. 1159-1168. ISSN 0893-6080

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To link to this item DOI: 10.1016/j.neunet.2009.07.014

Abstract/Summary

The bewildering complexity of cortical microcircuits at the single cell level gives rise to surprisingly robust emergent activity patterns at the level of laminar and columnar local field potentials (LFPs) in response to targeted local stimuli. Here we report the results of our multivariate data-analytic approach based on simultaneous multi-site recordings using micro-electrode-array chips for investigation of the microcircuitary of rat somatosensory (barrel) cortex. We find high repeatability of stimulus-induced responses, and typical spatial distributions of LFP responses to stimuli in supragranular, granular, and infragranular layers, where the last form a particularly distinct class. Population spikes appear to travel with about 33 cm/s from granular to infragranular layers. Responses within barrel related columns have different profiles than those in neighbouring columns to the left or interchangeably to the right. Variations between slices occur, but can be minimized by strictly obeying controlled experimental protocols. Cluster analysis on normalized recordings indicates specific spatial distributions of time series reflecting the location of sources and sinks independent of the stimulus layer. Although the precise correspondences between single cell activity and LFPs are still far from clear, a sophisticated neuroinformatics approach in combination with multi-site LFP recordings in the standardized slice preparation is suitable for comparing normal conditions to genetically or pharmacologically altered situations based on real cortical microcircuitry.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Centre for Integrative Neuroscience and Neurodynamics (CINN)
No Reading authors. Back catalogue items
ID Code:32505
Uncontrolled Keywords:Cortical microcircuits; Rodent; Somatosensory cortex; Multi-electrode array; Cortical layers; Impulse response; Principal component analysis; Information radius; Current-source density
Additional Information:The full text of this article is freely available via PMC using the link supplied in Related URLs
Publisher:Elsevier

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