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Measuring root traits in barley (Hordeum vulgare ssp vulgare and ssp spontaneum) seedlings using gel chambers, soil sacs and X-ray microtomography

Hargreaves, C. E., Gregory, P. J. and Bengough, A. G. (2009) Measuring root traits in barley (Hordeum vulgare ssp vulgare and ssp spontaneum) seedlings using gel chambers, soil sacs and X-ray microtomography. Plant and Soil, 316 (1-2). pp. 285-297. ISSN 0032-079X

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To link to this article DOI: 10.1007/s11104-008-9780-4

Abstract/Summary

Root characteristics of seedlings of five different barley genotypes were analysed in 2D using gel chambers, and in 3D using soil sacs that were destructively harvested and pots of soil that were assessed non-invasively using X-ray microtomography. After 5 days, Chime produced the greatest number of root axes (similar to 6) and Mehola significantly less (similar to 4) in all growing methods. Total root length was longest in GSH01915 and shortest in Mehola for all methods, but both total length and average root diameter were significantly larger for plants grown in gel chambers than those grown in soil. The ranking of particular growth traits (root number, root angular spread) of plants grown in gel plates, soil sacs and X-ray pots was similar, but plants grown in the gel chambers had a different order of ranking for root length to the soil-grown plants. Analysis of angles in soil-grown plants showed that Tadmore had the most even spread of individual roots and Chime had a propensity for non-uniform distribution and root clumping. The roots of Mehola were less well spread than the barley cultivars supporting the suggestion that wild and landrace barleys tend to have a narrower angular spread than modern cultivars. The three dimensional analysis of root systems carried out in this study provides insights into the limitations of screening methods for root traits and useful data for modelling root architecture.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Walker Institute for Climate System Research
Faculty of Science > School of Archaeology, Geography and Environmental Science
Interdisciplinary centres and themes > Centre for Food Security
Interdisciplinary centres and themes > Soil Research Centre
Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:3593
Uncontrolled Keywords:In situ root measurement - Root angles - Root length - Root traits - X-ray tomography
Additional Information:
Publisher:Springer

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