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Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis

Carnell-Morris, P., Tannetta, D., Siupa, A., Hole, P. and Dragovic, R. (2017) Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis. In: Extracellular Vesicles. Springer, pp. 153-173. ISBN 9781493972531

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To link to this item DOI: 10.1007/978-1-4939-7253-1_13

Abstract/Summary

Fluorescence nanoparticle tracking analysis (fl-NTA) allows for accurate sizing, counting, and phenotyping of extracellular vesicles (EV). Here, we present two protocols for the analysis of EVs using fl-NTA, highlighting the potential pitfalls and challenges. The first protocol utilizes CellMask Orange™ (CMO) as a general membrane marker to label EVs derived from plasma. The second protocol describes the use of a Qdot-conjugated antibody to identify syncytiotrophoblast (STB)-derived EVs. "Standard" preparations of STB-derived EVs enriched for either microvesicles (STBMV) or exosomes (STBEX), containing a known amount of EV positive for the STB specific antigen placental alkaline phosphatase (PLAP), were also used to optimize fl-NTA camera settings.

Item Type:Book or Report Section
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
ID Code:72285
Uncontrolled Keywords:Exosomes, Extracellular vesicles, Fluorescence nanoparticle tracking analysis, Microvesicles, Quantum dots
Publisher:Springer

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