Accessibility navigation


Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao)

Muller, E., Ullah, I. ORCID: https://orcid.org/0000-0002-9367-6741, Dunwell, J. ORCID: https://orcid.org/0000-0003-2147-665X, Daymond, A., Richardson, M., Allainguillaume, J. and Wetten, A. (2021) Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao). Scientific Reports, 11 (1). 8270. ISSN 2045-2322

[img]
Preview
Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.

3MB
[img] Text - Accepted Version
· Restricted to Repository staff only

843kB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1038/s41598-021-87690-1

Abstract/Summary

Theobroma cacao is one of the most economically important tropical trees, being the source of chocolate. As part of an ongoing study to understand the diversity of the badnavirus complex, responsible for the cacao swollen shoot virus disease in West Africa, evidence was found recently of virus-like sequences in asymptomatic cacao plants. The present study exploited the wealth of genomic resources in this crop, and combined bioinformatic, molecular, and genetic approaches to report for the first time the presence of integrated badnaviral sequences in most of the cacao genetic groups. These sequences, which we propose to name eTcBV for endogenous Theobroma cacao bacilliform virus, varied in type with each predominating in a specific genetic group. A diagnostic multiplex PCR method was developed to identify the homozygous or hemizygous condition of one specific insert, which was inherited as a single Mendelian trait. These data suggest that these integration events occurred before or during the species diversification in Central and South America, and prior to its cultivation in other regions. Such evidence of integrated sequences is relevant to the management of cacao quarantine facilities and may also aid novel methods to reduce the impact of such viruses in this crop.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
ID Code:97013
Publisher:Nature Publishing Group

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation