Accessibility navigation


DNA-templated self-assembly of bradykinin into bioactive nanofibrils

Lourenço, T. C., de Mello, L. R. ORCID: https://orcid.org/0000-0001-7630-5087, Icimoto, M. Y. ORCID: https://orcid.org/0000-0002-0746-5279, Bicev, R. N. ORCID: https://orcid.org/0000-0002-5951-9005, Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926, Castelletto, V., Nakaie, C. R. ORCID: https://orcid.org/0000-0001-7057-1990 and da Silva, E. R. ORCID: https://orcid.org/0000-0001-5876-2276 (2023) DNA-templated self-assembly of bradykinin into bioactive nanofibrils. Soft Matter, 19 (26). pp. 4869-4879. ISSN 1744-6848

[img]
Preview
Text - Accepted Version
· Please see our End User Agreement before downloading.

1MB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1039/d3sm00431g

Abstract/Summary

Bradykinin (BK) is a peptide hormone that plays a crucial role in blood pressure control, regulates inflammation in the human body, and has recently been implicated in the pathophysiology of COVID-19. In this study, we report a strategy for fabricating highly ordered 1D nanostructures of BK using DNA fragments as a template for self-assembly. We have combined synchrotron small-angle X-ray scattering and high-resolution microscopy to provide insights into the nanoscale structure of BK-DNA complexes, unveiling the formation of ordered nanofibrils. Fluorescence assays hint that BK is more efficient at displacing minor-groove binders in comparison with base-intercalant dyes, thus, suggesting that interaction with DNA strands is mediated by electrostatic attraction between cationic groups at BK and the high negative electron density of minor-grooves. Our data also revealed an intriguing finding that BK-DNA complexes can induce a limited uptake of nucleotides by HEK-293t cells, which is a feature that has not been previously reported for BK. Moreover, we observed that the complexes retained the native bioactivity of BK, including the ability to modulate Ca2+ response into endothelial HUVEC cells. Overall, the findings presented here demonstrate a promising strategy for the fabrication of fibrillar structures of BK using DNA as a template, which keep bioactivity features of the native peptide and may have implications in the development of nanotherapeutics for hypertension and related disorders.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:112395
Uncontrolled Keywords:Condensed Matter Physics, General Chemistry
Publisher:Royal Society of Chemistry (RSC)

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation