Accessibility navigation

In vitro evaluation of the fermentation properties and potential prebiotic activity of Agave fructans

Gomez, E., Tuohy, K.M., Gibson, G.R., Klinder, A. and Costabile, A. (2010) In vitro evaluation of the fermentation properties and potential prebiotic activity of Agave fructans. Journal of Applied Microbiology, 108 (6). pp. 2114-2121. ISSN 1364-5072

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1111/j.1365-2672.2009.04617.x


Aims: This study was carried out to evaluate in vitro the fermentation properties and the potential prebiotic activity of Agave-fructans extracted from Agave tequilana (Predilife). Methods and Results: Five different commercial prebiotics were compared using 24-h pH-controlled anaerobic batch cultures inoculated with human faecal slurries. Measurement of prebiotic efficacy was obtained by comparing bacterial changes, and the production of short-chain fatty acids (SCFA) was also determined. Effects upon major groups of the microbiota were monitored over 24 h incubations by fluorescence in situ hybridization. SCFA were measured by HPLC. Fermentation of the Agave fructans (Predilife) resulted in a large increase in numbers of bifidobacteria and lactobacilli. Conclusions: Under the in vitro conditions used, this study has shown the differential impact of Predilife on the microbial ecology of the human gut. Significance and Impact of the Study: This is the first study reporting of a potential prebiotic mode of activity for Agave fructans investigated which significantly increased populations of bifidobacteria and lactobacilli compared to cellulose used as a control.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:13372
Uncontrolled Keywords:Agave, batch culture system, gut microflora, inulin, prebiotic activity , OLIGONUCLEOTIDE PROBES, SITU HYBRIDIZATION, HUMAN FECES, OLIGOSACCHARIDES, OLIGOFRUCTOSE, VOLUNTEERS, MODULATION, INULIN
Publisher:The Society for Applied Microbiology

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation