Accessibility navigation

Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+ - and K+ -permeable conductance in root cells

Laohavisit, A., Shang, Z., Rubio, L., Cuin, T. A., Very, A.-A., Wang, A., Mortimer, J. C., Macpherson, N., Coxon, K. M., Battey, N. H., Brownlee, C., Park, O. K., Sentenac, H., Shabala, S., Webb, A. A. R. and Davies, J. M. (2012) Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+ - and K+ -permeable conductance in root cells. The Plant Cell, 24 (4). pp. 1522-1533. ISSN 1040-4651

Text - Published Version
· Please see our End User Agreement before downloading.


It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1105/tpc.112.097881


Plant cell growth and stress signaling require Ca2+ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH_. In root cells, extracellular OH_ activates a plasma membrane Ca2+-permeable conductance that permits Ca2+ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca2+-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH_-activated Ca2+- and K+-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca2+ in response to OH_. An OH_-activated Ca2+ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca2+ in plants.

Item Type:Article
Divisions:Life Sciences > School of Biological Sciences > Ecology and Evolutionary Biology
ID Code:27903
Publisher:American Society of Plant Biologists


Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation