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Use of TD-GC-TOF-MS to assess volatile composition during post harvest storage in seven accessions of rocket salad (Eruca sativa)

Bell, L. ORCID:, Spadafora, N. D., Mueller, C. T., Wagstaff, C. ORCID: and Rogers, H. j. (2016) Use of TD-GC-TOF-MS to assess volatile composition during post harvest storage in seven accessions of rocket salad (Eruca sativa). Food Chemistry, 194. pp. 626-636. ISSN 0308-8146

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To link to this item DOI: 10.1016/j.foodchem.2015.08.043


An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD- GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf-life.

Item Type:Article
Divisions:Interdisciplinary centres and themes > Centre for Food Security
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
ID Code:41354
Uncontrolled Keywords:Eruca sativa; Diplotaxis tenuifolia; Brassicaceae; rocket salad; rucola; gas 28 chromatography mass spectrometry; volatile organic chemicals; isothiocyanates.


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