Accessibility navigation


The dynamics of protein body formation in developing wheat grain

Moore, K. L., Tosi, P. ORCID: https://orcid.org/0000-0003-4171-6120, Palmer, R., Hawkesford, M. J., Grovenor, C. R. M. and Shewry, P. R. (2016) The dynamics of protein body formation in developing wheat grain. Plant Biotechnology Journal, 14 (9). PBI-00553-2015.R1. ISSN 1467-7652

[img]
Preview
Text (Open access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.

1MB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1111/pbi.12549

Abstract/Summary

Wheat is a major source of protein in the diets of humans and livestock but we know little about the mechanisms that determine the patterns of protein synthesis in the developing endosperm. We have used a combination of enrichment with 15N glutamine and NanoSIMS imaging to establish that the substrate required for protein synthesis is transported radially from its point of entrance in the endosperm cavity across the starchy endosperm tissues, before becoming concentrated in the cells immediately below the aleurone layer. This transport occurs continuously during grain development but may be slower in the later stages. Although older starchy endosperm cells tend to contain larger protein deposits formed by the fusion of small protein bodies, small highly enriched protein bodies were also oberved in the same cell m. This suggest a continuous process of protein body initiation, in both older and younger starchy endosperm cells and in all regions of the tissue. Immunolabelling with specific antibodies shows that the patterns of enrichment are not related to the contents of gluten proteins in the protein bodies. In addition to providing new information on the dynamics of protein deposition, the study demonstrates the wider utility of NanoSIMS and isotope labeling for studying complex developmental processes in plant tissues.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
ID Code:55810
Publisher:Wiley

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation