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Protein precipitation behavior of condensed tannins from Lotus pedunculatus and Trifolium repens with different mean degrees ofpPolymerization

Zeller, W. E., Sullivan, M. L., Mueller-Harvey, I., Grabber, J. H., Ramsay, A., Drake, C. and Brown, R. H. (2015) Protein precipitation behavior of condensed tannins from Lotus pedunculatus and Trifolium repens with different mean degrees ofpPolymerization. Journal of Agricultural and Food Chemistry, 63 (4). pp. 1160-1168. ISSN 1520-5118

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To link to this item DOI: 10.1021/jf504715p

Abstract/Summary

The precipitation of bovine serum albumin (BSA), lysozyme (LYS), and alfalfa leaf protein (ALF) by two large- and two medium-sized condensed tannin (CT) fractions of similar flavan-3-ol subunit composition is described. CT fractions isolated from white clover flowers and big trefoil leaves exhibited high-purity profiles by 1D/2D NMR and purities >90% (determined by thiolysis). At pH 6.5, large CTs with a mean degree of polymerization (mDP) of ∼18 exhibited similar protein precipitation behaviors and were significantly more effective than medium CTs (mDP ∼9). Medium CTs exhibited similar capacities to precipitate ALF or BSA, but showed small but significant differences in their capacity to precipitate LYS. All CTs precipitated ALF more effectively than BSA or LYS. Aggregation of CT–protein complexes likely aided precipitation of ALF and BSA, but not LYS. This study, one of the first to use CTs of confirmed high purity, demonstrates that the mDP of CTs influences protein precipitation efficacy.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences > Animal, Dairy and Food Chain Sciences (ADFCS)- DO NOT USE
ID Code:73891
Uncontrolled Keywords:General Chemistry, General Agricultural and Biological Sciences
Publisher:American Chemical Society (ACS)

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