Draft genome sequence of a multidrug-resistant CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli isolate (ST131-O25b-H30) from Pakistan exhibiting high potential virulence
Rafaque, Z., Dasti, J. I. and Andrews, S. C.
It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1016/j.jgar.2018.10.001 Abstract/SummaryObjectives: Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTI). The pandemic ST131 clonal type is associated with multidrug resistance and virulence. We report the first draft genome of an ST131-O25b-H30 strain from Pakistan, isolated from a patient with community-acquired UTI. Methods: Next-generation sequencing was performed using MiSeq and HiSeq 2500 platforms. De novo assembly of the reads was performed using SPAdes (v 3.11.). The genomic features were determined with PATRIC and RAST tool kit. Results: The 5,327,975 bp draft genome sequence has 5433 CDS and 82 tRNAs, an array of resistance genes (blaCTXM-15, blaOXA-1, blaCMY-2, sul2, catB, dfrA17, mph(A)), one class 1 integron, 77 IS elements, one transposon (Tn3-like), multiple virulence markers and seven intact prophage loci. Conclusion: In conclusion, the genome sequence of this new UPEC isolate from Pakistan provides novel insight into the genetic attributes of an epidemic clone associated with high level of resistance and virulence.
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