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Induction and growth of 'microspore-derived' embryos of Brassica napus ssp. oleifera

Dunwell, J. M., Cornish, M., De Courcel, A. G. L. and Middlefell-Williams, J. E. (1983) Induction and growth of 'microspore-derived' embryos of Brassica napus ssp. oleifera. Journal of Experimental Botany, 34 (12). pp. 1768-1778. ISSN 0022-0957

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To link to this item DOI: 10.1093/jxb/34.12.1768


Three cultivars of spring rape (Brassica napus ssp. oleifera), Tower, Willi and Duplo, were used for a study of induction and growth of 'microspore-derived' embryos, Buds, 2.0 mm in length, containing uninucleate microspores were harvested and stored for 14 d at 4 °C in darkness. Anthers were then removed and cultured on a liquid medium based upon that of Murashige and Skoog and containing 8% sucrose, 0.5 mg l-1 naphthylacetic acid and 0-05 mg l-1 benzylaminopurine. Cultures were pre-incubated at 35 °C for 0-3 d and then incubated at 30 °C. After a total of 42 d incubation, cultures were scored for the presence of macroscopic embryos (1-2 mm in length) and for the presence of anthers containing abortive embryoids which had not developed further. The cultivars differed greatly in terms both of the frequency of anthers showing induced embryoids and of the final yield of embryos. Tower showed the highest frequency of induction (maximum 38% of cultured anthers with induced embryoids) whereas the highest yield (equivalent to 1 • 1 embryo per cultured anther) was obtained from anthers of the cv. Duplo after a 3 d treatment at 35 °C. Yields from the other cultivars were much lower and were relatively unaffected by the 35 °C treatment.

Item Type:Article
Divisions:Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
ID Code:86952
Publisher:Oxford University Press

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