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Synthesis of fluorescently-labelled poly(2-ethyl-2-oxazoline)-protected gold nanoparticles

Nurgaziyeva, E. K., Kudaibergenov, S. E., Mun, G. A. and Khutoryanskiy, V. V. (2021) Synthesis of fluorescently-labelled poly(2-ethyl-2-oxazoline)-protected gold nanoparticles. Chemical Bulletin of Kazakh National University, 100 (1). pp. 12-20. ISSN 2312-7554

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To link to this item DOI: 10.15328/cb1185

Abstract/Summary

Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular weights (Mw = 5, 50, 200 and 500 kDa) were synthesised and characterised by dynamic light scattering, nanoparticle tracking analysis, zeta potential measurement and transmission electron microscopy. It was established that the use of POZ with 50 kDa resulted in formation of GNPs with low polydispersity while POZ with greater molecular weights led to formation of more polydisperse GNPs. Fluorescent labelling of these nanoparticles was achieved through their reaction with polyethyleneglycol dithiol (8-12 kDa) as a linker molecule with subsequent reaction with 6-(iodoacetamido) fluorescein. The fluorescent nature of obtained GNPs was confirmed by the appearance of the fluorescence peak at 510 nm that is typical for fluorescein molecules and glowing of the aqueous solution under the UV irradiaton. The fluorescently-labelled GNPs are promising tool in biomedical application to monitor the biological systems using fluorescent microscopy.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
ID Code:97705
Publisher:Kazakh National University

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