Article

Meltem Sarıyer, R., Gill, K. K., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Reis, N. M., Jones, C. I. ORCID: https://orcid.org/0000-0001-7537-1509 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2024) Looping flexible fluoropolymer microcapillary film extends analysis times for vertical microfluidic blood testing. Sensors, 24 (18). 5870. ISSN 1424-8220 doi: https://doi.org/10.3390/s24185870

Sariyer, R. M., Gill, K., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Hodge, D., Reis, N. M., Jones, C. I. ORCID: https://orcid.org/0000-0001-7537-1509 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2023) Time- and distance-resolved robotic imaging of fluid flow in vertical microfluidic strips: a new technique for quantitative, multiparameter measurement of global haemostasis. Sensors and Diagnostics, 2 (6). pp. 1623-1637. ISSN 2635-0998 doi: https://doi.org/10.1039/D3SD00162H

Sariyer, R. M., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X and Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637 (2023) Open hardware for microfluidics: exploiting Raspberry Pi singleboard computer and camera systems for customisable laboratory instrumentation. Biosensors, 13 (10). 948. ISSN 2079-6374 doi: https://doi.org/10.3390/bios13100948

Long, M. M. ORCID: https://orcid.org/0000-0003-3646-2218, Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2023) Dilution reduces sample matrix effects for rapid, direct, and miniaturised phenotypic antibiotic susceptibility tests for bovine mastitis. Antibiotics, 12 (9). 1363. ISSN 2079-6382 doi: https://doi.org/10.3390/antibiotics12091363

Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Pivetal, J., Hayward, J., Kidd, S. P., Lam, H., Diep, T., Gill, K., Woodward, M., Reis, N. M. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2023) Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and usability for detection of urinary tract infections in human urine. Sensors and Diagnostics, 2 (3). ISSN 2635-0998 doi: https://doi.org/10.1039/d2sd00138a

Sarıyer, R. M. ORCID: https://orcid.org/0000-0002-2155-653X, Gill, K., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Hodge, D., Reis, N. M., Jones, C. I. ORCID: https://orcid.org/0000-0001-7537-1509 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2023) Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis. British Journal of Pharmacy, 8 (2). ISSN 2058-8356 doi: https://doi.org/10.5920/bjpharm.1365

Walker, S. ORCID: https://orcid.org/0000-0001-5942-1836, Bravo, J. ORCID: https://orcid.org/0000-0002-1013-8412, Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X, Hart, J. and Little, G. (2022) Instructions for COVID-19 self-tests: what parts of the test are the most difficult to get right and how can information design help? Information Design Journal, 27 (1). pp. 126-139. ISSN 1569-979X doi: https://doi.org/10.1075/idj.22011.wal

Diep, T. T., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Bizley, S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Rapid bacterial motility monitoring using inexpensive 3D-printed openflexure microscopy allows microfluidic antibiotic susceptibility testing. Micromachines, 13 (11). 1974. ISSN 2072-666X doi: https://doi.org/10.3390/mi13111974

Long, M. M., Diep, T. T., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Ross, M. J. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) PiRamid: A compact Raspberry Pi imaging box to automate small-scale time-lapse digital analysis, suitable for laboratory and field use. HardwareX, 12. e00377. ISSN 24680672 doi: https://doi.org/10.1016/j.ohx.2022.e00377

Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Saiprom, N., Rafaque, Z., Imtiaz, W., Chantratita, N., Runcharoen, C., Thammachote, J., Anun, S., Peacock, S. J., Ray, P. ORCID: https://orcid.org/0000-0001-8375-8279, Andrews, S. ORCID: https://orcid.org/0000-0003-4295-2686 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Miniaturised broth microdilution for simplified antibiotic susceptibility testing of Gram negative clinical isolates using microcapillary devices. Analyst (15). pp. 3385-3614. ISSN 0003-2654 doi: https://doi.org/10.1039/D2AN00305H

Diep, T. T. ORCID: https://orcid.org/0000-0001-7166-6369, Bizley, S. ORCID: https://orcid.org/0000-0002-4971-777X and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) 3D-printed dip slides miniaturize bacterial identification and antibiotic susceptibility tests allowing direct mastitis sample analysis. Micromachines, 13 (6). p. 941. ISSN 2072-666X doi: https://doi.org/10.3390/mi13060941

Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Sirivisoot, S. ORCID: https://orcid.org/0000-0002-5809-6936, Jegouic, S., Prommool, T., Luangaram, P., Srisawat, C., Sriraksa, K., Limpitikul, W., Mairiang, D. ORCID: https://orcid.org/0000-0002-2743-0145, Malasit, P., Avirutnan, P. ORCID: https://orcid.org/0000-0003-0053-3045, Puttikhunt, C. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Smartphone multiplex microcapillary diagnostics using Cygnus: development and evaluation of rapid serotype-specific NS1 detection with dengue patient samples. PLOS Neglected Tropical Diseases, 16 (4). e0010266. ISSN 1935-2735 doi: https://doi.org/10.1371/journal.pntd.0010266

Diep, T. T. ORCID: https://orcid.org/0000-0001-7166-6369, Ray, P. P. ORCID: https://orcid.org/0000-0001-8375-8279 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Methods for rapid prototyping novel labware: using CAD and desktop 3D printing in the microbiology laboratory. Letters in Applied Microbiology, 74 (2). pp. 247-257. ISSN 1472-765X doi: https://doi.org/10.1111/lam.13615

Donmez, S. I., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Osborn, H. M. I. ORCID: https://orcid.org/0000-0002-0683-0457, Reis, N. M. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques. Lab on a Chip, 22 (15). pp. 2820-2831. ISSN 1473-0197 doi: https://doi.org/10.1039/d2lc00280a

Reis, N. M. ORCID: https://orcid.org/0000-0002-8706-6998, Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Jegouic, S. M., Gill, K. K., Sirivisoot, S., Howard, S., Kempe, J., Bola, S., Al-Hakeem, K., Jones, I. M. ORCID: https://orcid.org/0000-0002-7738-2516, Prommool, T., Luangaram, P., Avirutnan, P., Puttikhunt, C. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Gravity-driven microfluidic siphons: fluidic characterization and application to quantitative immunoassays. ACS Sensors, 6 (12). pp. 4338-4348. ISSN 2379-3694 doi: https://doi.org/10.1021/acssensors.1c01524

Dobia, A. M., Ryan, K. M., BaHammam, A. S., Abutaleb, M. H., Alwadani, R. Y. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) The use of hypnotics for Insomnia Disorder in Saudi Arabia: a survey of patients’ experiences and perceptions. Sleep and Vigilance, 5 (2). pp. 235-244. ISSN 2510-2265 doi: https://doi.org/10.1007/s41782-021-00151-7

Diep, T. T., Bizley, S., Ray, P. P. ORCID: https://orcid.org/0000-0001-8375-8279 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) MicroMI: a portable microbiological mobile incubator that uses inexpensive lithium power banks for field microbiology. HardwareX, 10. ISSN 24680672 doi: https://doi.org/10.1016/j.ohx.2021.e00242

Needs, S. ORCID: https://orcid.org/0000-0003-3407-9637, Osborn, H. ORCID: https://orcid.org/0000-0002-0683-0457 and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Counting bacteria in microfluidic devices: smartphone compatible ‘dip-and-test’ viable cell quantitation using resazurin amplified detection in microliter capillary arrays. Journal of Microbiological Methods, 187. 106199. ISSN 0167-7012 doi: https://doi.org/10.1016/j.mimet.2021.106199

Jegouic, S. M., Jones, I. M. ORCID: https://orcid.org/0000-0002-7738-2516 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Affordable mobile microfluidic diagnostics: minimum requirements for smartphones and digital imaging for colorimetric and fluorometric anti-dengue and anti-SARS-CoV-2 antibody detection. Wellcome Open Research, 6. 57. ISSN 2398-502X doi: https://doi.org/10.12688/wellcomeopenres.16628.1

Barbosa, A. I., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X and Reis, N. M. (2021) Antibody surface coverage drives matrix interference in microfluidic capillary immunoassays. ACS Sensors, 6 (7). pp. 2682-2690. ISSN 2379-3694 doi: https://doi.org/10.1021/acssensors.1c00704

Needs, S. ORCID: https://orcid.org/0000-0003-3407-9637, Donmez, S. I. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Direct microfluidic antibiotic resistance testing in urine with smartphone capture: significant variation in sample matrix interference between individual human urine samples. RSC Advances, 11 (60). pp. 38258-38263. ISSN 2046-2069 doi: https://doi.org/10.1039/D1RA06867A

Dönmez, S. İ., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Osborn, H. M. I. ORCID: https://orcid.org/0000-0002-0683-0457 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Label-free smartphone quantitation of bacteria by darkfield imaging of light scattering in fluoropolymer micro capillary film allows portable detection of bacteriophage lysis. Sensors and Actuators B: Chemical, 323. p. 128645. ISSN 0925-4005 doi: https://doi.org/10.1016/j.snb.2020.128645

Needs, S. ORCID: https://orcid.org/0000-0003-3407-9637, Donmez, S. I., Bull, S. ORCID: https://orcid.org/0000-0001-5129-1731, McQuaid, C., Osborn, H. M. I. ORCID: https://orcid.org/0000-0002-0683-0457 and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Challenges in microfluidic and point-of-care phenotypic antimicrobial resistance tests. Frontiers in Mechanical Engineering, 6. ISSN 2297-3079 doi: https://doi.org/10.3389/fmech.2020.00073

Needs, S. ORCID: https://orcid.org/0000-0003-3407-9637, Bull, S. ORCID: https://orcid.org/0000-0001-5129-1731, Bravo, J. ORCID: https://orcid.org/0000-0002-1013-8412, Walker, S. ORCID: https://orcid.org/0000-0001-5942-1836, Little, G., Hart, J. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Remote videolink observation of model home sampling and home testing devices to simplify usability studies for point-of-care diagnostics. Wellcome Open Research, 5 (174). ISSN 2398-502X doi: https://doi.org/10.12688/wellcomeopenres.16105.1

Dobia, A., Ryan, K., BaHammam, A. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2019) Using benzodiazepines and Z-Drugs for managing primary insomnia in adults in Saudi Arabia: an e-Delphi study to aid the development of clinical guidelines. Sleep and Breathing, 23 (4). pp. 1133-1139. ISSN 1520-9512 doi: https://doi.org/10.1007/s11325-019-01794-7

Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Diep, T. T., Bull, S. P. ORCID: https://orcid.org/0000-0001-5129-1731, Lindley-Decaire, A., Ray, P. ORCID: https://orcid.org/0000-0001-8375-8279 and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2019) Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology. PLOS ONE, 14 (11). e0224878. ISSN 1932-6203 doi: https://doi.org/10.1371/journal.pone.0224878

Dobia, A., Ryan, K., Abutaleb, M. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2019) Perceptions of physicians in Saudi Arabia on the use of international clinical guidelines for managing primary insomnia. PLoS ONE, 14 (8). e0220960. ISSN 1932-6203 doi: https://doi.org/10.1371/journal.pone.0220960

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Barbosa, A. I., Gehlot, P., Sidapra, K., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X and Reis, N. M. (2015) Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70. pp. 5-14. ISSN 0956-5663 doi: https://doi.org/10.1016/j.bios.2015.03.006

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Cook, M. T., Saratoon, T., Tzortzis, G., Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X, Charalampopoulos, D. ORCID: https://orcid.org/0000-0003-1269-8402 and Khutoryanskiy, V. V. ORCID: https://orcid.org/0000-0002-7221-2630 (2013) CLSM method for the dynamic observation of pH change within polymer matrices for oral delivery. Biomacromolecules, 14 (2). pp. 387-393. ISSN 1525-7797 doi: https://doi.org/10.1021/bm301569r

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Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Reis, N. M., Slater, N. K. H. and Mackley, M. R. (2011) A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. Lab on a Chip, 11 (24). pp. 4267-4273. ISSN 1473-0197 doi: https://doi.org/10.1039/C0LC00357C

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Cheeks, M. C., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Arnot, C. J. and Slater, N. K.H. (2009) Gene transfection of HEK cells on supermacroporous polyacrylamide monoliths: a comparison of transient and stable recombinant protein expression in perfusion culture. New Biotechnology, 26 (6). pp. 289-299. ISSN 1871-6784 doi: https://doi.org/10.1016/j.nbt.2009.08.006

Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X and Slater, N. K.H. (2009) Protection of live bacteria from bile acid toxicity using bile acid adsorbing resins. Vaccine, 27 (29). pp. 3897-3903. ISSN 0264-410X doi: https://doi.org/10.1016/j.vaccine.2009.04.006

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Carr, J. M., Carrasco, M. J., Thaventhiran, J. E. D., Bambrough, P. J., Kraman, M., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Al-Shamkhani, A. and Fearon, D. T. (2006) CD27 mediates interleukin-2-independent clonal expansion of the CD8+ T cell without effector differentiation. Proceedings of the National Academy of Sciences, 103 (51). p. 19454. ISSN 0027-8424 doi: https://doi.org/10.1073/pnas.0609706104

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Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Chaussabel, D., Tomlinson, S., Schulz, O., Sher, A. and Reis e Sousa, C. (2003) Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns. The Journal of Immunology, 171 (1). pp. 47-60. ISSN 0022-1767 doi: https://doi.org/10.4049/jimmunol.171.1.47

Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Diebold, S. S., Slack, E. M. C., Tomizawa, H., Hemmi, H., Kaisho, T., Akira, S. and Sousa, C. R. e. (2003) Toll-like receptor expression in murine DC subsets: lack of TLR7 expression by CD8α+ DC correlates with unresponsiveness to imidazoquinolines. European Journal of Immunology, 33 (4). p. 827. ISSN 00142980 doi: https://doi.org/10.1002/eji.200323797

Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Manickasingham, S. P., Sporri, R., Diebold, S. S., Schulz, O., Sher, A., Kaisho, T., Akira, S. and Reis e Sousa, C. (2002) Microbial Recognition Via Toll-Like Receptor-Dependent and -Independent Pathways Determines the Cytokine Response of Murine Dendritic Cell Subsets to CD40 Triggering. The Journal of Immunology, 169 (7). pp. 3652-3660. ISSN 0022-1767 doi: https://doi.org/10.4049/jimmunol.169.7.3652

Schulz, O., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X, Schito, M., Aliberti, J., Manickasingham, S., Sher, A. and Reis e Sousa, C. (2000) CD40 Triggering of Heterodimeric IL-12 p70 Production by Dendritic Cells In Vivo Requires a Microbial Priming Signal. Immunity, 13 (4). p. 453. ISSN 10747613 doi: https://doi.org/10.1016/S1074-7613(00)00045-5

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Walker, S. ORCID: https://orcid.org/0000-0001-5942-1836, Bravo, J. ORCID: https://orcid.org/0000-0002-1013-8412 and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2024) Testing, testing – what about the instructions? In: Cooper, F. and Fitzgerald, D. (eds.) Knowing COVID-19: The Pandemic and Beyond. Manchester University Press, Manchester, pp. 60-85. ISBN 9781526178640 doi: https://doi.org/10.7765/9781526178657.00009

Reis, N. M., Alves, I., Pereira, F., Jegouic, S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Point-of-need detection with smartphone. In: Hussain, C. (ed.) Smartphone-Based Detection Devices. Elsevier, pp. 311-362. ISBN 9780128236963 doi: https://doi.org/10.1016/B978-0-12-823696-3.00016-7

Edwards, A.D. ORCID: https://orcid.org/0000-0003-2369-989X (2013) What have dendritic cells ever done for adjuvant design? Cellular and molecular methods for the rational development of vaccine adjuvants. In: Flower , D. R. and Perrie, Y. (eds.) Immunomic Discovery of Adjuvants and Candidate Subunit Vaccines. Immunomics reviews (5). Springer, New York , pp. 131-154. ISBN 978-1-4614-5070-2 doi: https://doi.org/10.1007/978-1-4614-5070-2_8

Jopeace, A.L., Howard, C.B., Murton, B.L., Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X and Monie, T.P. (2012) Pattern recognition receptors and infectious diseases. In: Insight and control of infectious disease in global scenario. InTech Open. ISBN 9789535103196

Report

Walker, S. ORCID: https://orcid.org/0000-0001-5942-1836, Bravo, J. ORCID: https://orcid.org/0000-0002-1013-8412 and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X, (2022) User-friendly point-of-use instructions for home use diagnostic tests. Other. University of Reading doi: https://doi.org/10.17864/1947.000419

Bull, S. ORCID: https://orcid.org/0000-0001-5129-1731, Needs, S. ORCID: https://orcid.org/0000-0003-3407-9637 and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X, (2020) Building open lab hardware to tackle antimicrobial resistance. Open Research Case Studies. Report. University of Reading doi: https://doi.org/10.17864/1926.89600

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