Accessibility navigation


The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms

Jing, R., Bolshakov, V. and Flavell, A. J. (2007) The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms. Nat Protoc, 2 (1). pp. 168-77. ISSN 1750-2799

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

Official URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract/Summary

The tagged microarray marker (TAM) method allows high-throughput differentiation between predicted alternative PCR products. Typically, the method is used as a molecular marker approach to determining the allelic states of single nucleotide polymorphisms (SNPs) or insertion-deletion (indel) alleles at genomic loci in multiple individuals. Biotin-labeled PCR products are spotted, unpurified, onto a streptavidin-coated glass slide and the alternative products are differentiated by hybridization to fluorescent detector oligonucleotides that recognize corresponding allele-specific tags on the PCR primers. The main attractions of this method are its high throughput (thousands of PCRs are analyzed per slide), flexibility of scoring (any combination, from a single marker in thousands of samples to thousands of markers in a single sample, can be analyzed) and flexibility of scale (any experimental scale, from a small lab setting up to a large project). This protocol describes an experiment involving 3,072 PCRs scored on a slide. The whole process from the start of PCR setup to receiving the data spreadsheet takes 2 d.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10024
Uncontrolled Keywords:Biotin, DNA Transposable Elements/*genetics, Fluorescence, Genetic Markers/*genetics, Microarray Analysis/*methods, Oligonucleotides, Polymerase Chain Reaction/*methods, Polymorphism, Single Nucleotide/*genetics

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation