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Thioquinolobactin, a pseudomonas siderophore with antifungal and anti-pythium activity

Matthijs, S., Tehrani, K. A., Laus, G., Jackson, R. W., Cooper, R. M. and Cornelis, P. (2007) Thioquinolobactin, a pseudomonas siderophore with antifungal and anti-pythium activity. Environmental Microbiology, 9 (2). pp. 425-34. ISSN 1462-2912

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To link to this article DOI: 10.1111/j.1462-2920.2006.01154.x

Abstract/Summary

Under conditions of iron limitation Pseudomonas fluorescens ATCC 17400 produces two siderophores, pyoverdine, and a second siderophore quinolobactin, which itself results from the hydrolysis of the unstable molecule 8-hydroxy-4-methoxy-2-quinoline thiocarboxylic acid (thioquinolobactin). Pseudomonas fluorescens ATCC 17400 also displays a strong in vitro antagonism against the Oomycete Pythium, which is repressed by iron, suggesting the involvement of a siderophore(s). While a pyoverdine-negative mutant retains most of its antagonism, a thioquinolobactin-negative mutant only slowed-down Pythium growth, and a double pyoverdine-, thioquinolobactin-negative mutant, which does not produce any siderophore, totally lost its antagonism against Pythium. The siderophore thioquinolobactin could be purified and identified from spent medium and showed anti-Pythium activity, but it was quickly hydrolysed to quinolobactin, which we showed has no antimicrobial activity. Analysis of antagonism-affected transposon mutants revealed that genes involved in haem biosynthesis and sulfur assimilation are important for the production of thioquinolobactin and the expression of antagonism.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
Interdisciplinary centres and themes > Soil Research Centre
ID Code:10045
Uncontrolled Keywords:Antifungal Agents/chemistry/isolation & purification/*pharmacology, DNA Transposable Elements, Heme/biosynthesis, Hydrolysis, Molecular Sequence Data, Mutation, Oligopeptides/genetics/metabolism, Phenotype, Pseudomonas fluorescens/*chemistry/genetics/metabolism, Pythium/*drug effects/growth & development, Quinolines/chemistry/isolation & purification/metabolism/*pharmacology, Siderophores/chemistry/isolation & purification/*pharmacology, Sulfur/metabolism

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