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Osmotic upshift transiently inhibits uptake via ABC transporters in gram-negative bacteria

Fox, M. A., White, J. P., Hosie, A. H. F., Lodwig, E. M. and Poole, P. S. (2006) Osmotic upshift transiently inhibits uptake via ABC transporters in gram-negative bacteria. Journal of Bacteriology, 188 (14). pp. 5304-5307. ISSN 0021-9193

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To link to this item DOI: 10.1128/jb.00262-06

Abstract/Summary

ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media. Measurement of nutrient cycling in isolated pea bacteroids is impeded by this effect.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10158
Uncontrolled Keywords:PEA ROOT-NODULES, LEGUMINOSARUM BV VICIAE, AMINO-ACID PERMEASE, RHIZOBIUM-LEGUMINOSARUM, MYOINOSITOL CATABOLISM, NITROGEN-FIXATION, GLUTAMATE, IDENTIFICATION, ALANINE, EFFLUX

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