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Significant redox insensitivity of the functions of the SARS-CoV spike glycoprotein - Comparison with HIV envelope

Lavillette, D., Barbouche, R., Yao, Y. X., Boson, B., Cosset, F. L., Jones, I. M. and Fenouillet, E. (2006) Significant redox insensitivity of the functions of the SARS-CoV spike glycoprotein - Comparison with HIV envelope. The Journal of Biological Chemistry, 281 (14). pp. 9200-9204. ISSN 1083-351X

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To link to this article DOI: 10.1074/jbc.M512529200

Abstract/Summary

The capacity of the surface glycoproteins of enveloped viruses to mediate virus/cell binding and membrane fusion requires a proper thiol/disulfide balance. Chemical manipulation of their redox state using reducing agents or free sulfhydryl reagents affects virus/cell interaction. Conversely, natural thiol/disulfide rearrangements often occur during the cell interaction to trigger fusogenicity, hence the virus entry. We examined the relationship between the redox state of the 20 cysteine residues of the SARS-CoV (severe acute respiratory syndrome coronavirus) Spike glycoprotein S1 subdomain and its functional properties. Mature S1 exhibited similar to 4 unpaired cysteines, and chemically reduced S1 displaying up to similar to 6 additional unpaired cysteines still bound ACE2 and enabled fusion. In addition, virus/cell membrane fusion occurred in the presence of sulfhydryl-blocking reagents and oxidoreductase inhibitors. Thus, in contrast to various viruses including HIV (human immunodeficiency virus) examined in parallel, the functions of the SARS-CoV Spike glycoprotein exhibit a significant and surprising independence of redox state, which may contribute to the wide host range of the virus. These data suggest clues for molecularly engineering vaccine immunogens.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10187
Uncontrolled Keywords:RESPIRATORY SYNDROME CORONAVIRUS, PROTEIN-DISULFIDE-ISOMERASE, ANGIOTENSIN-CONVERTING ENZYME-2, MEMBRANE-FUSION, VIRUS ENVELOPE, CELL-FUSION, NEUTRALIZING ANTIBODY, MEDIATED REDUCTION, RECEPTOR, INFECTION

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