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Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome

Mauchline, T. H., Fowler, J. E., East, A. K., Sartor, A. L., Zaheer, R., Hosie, A. H. F., Poole, P. S. and Finan, T. M. (2006) Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome. Proceedings of the National Academy of Sciences of the United States of America, 103 (47). pp. 17933-17938. ISSN 0027-8424

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To link to this item DOI: 10.1073/pnas.0606673103


The number of solute-binding protein-dependent transporters in rhizobia is dramatically increased compared with the majority of other bacteria so far sequenced. This increase may be due to the high affinity of solute-binding proteins for solutes, permitting the acquisition of a broad range of growth-limiting nutrients from soil and the rhizosphere. The transcriptional induction of these transporters was studied by creating a suite of plasmid and integrated fusions to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to approximate to 47% of the ABC uptake systems and 53% of the TRAP transporters in S. meliloti. Of these transport systems, 64 are previously uncharacterized in Rhizobia and 24 were induced by solutes not known to be transported by ABC- or TRAP-uptake systems in any organism. This study provides a global expression map of one of the largest transporter families (transportome) and an invaluable tool to both understand their solute specificity and the relationships between members of large paralogous families.

Item Type:Article
Divisions:Faculty of Life Sciences > School of Biological Sciences
Interdisciplinary centres and themes > Soil Research Centre
ID Code:10192
Additional Information:Conference proceedings: Proceedings on the National Academy of Sciences USA 2006 ISI Document Delivery No.: 111PG Times Cited: 31 Cited Reference Count: 49 Cited References: BARDIN S, 1996, J BACTERIOL, V178, P4540 BARNETT MJ, 2004, P NATL ACAD SCI USA, V101, P16636, DOI 10.1073/pnas.0407269101 BECKER A, 2004, MOL PLANT MICROBE IN, V17, P292 BLATTNER FR, 1997, SCIENCE, V277, P1453 BONCOMPAGNI E, 2000, J BACTERIOL, V182, P3717 BRINGHURST RM, 2001, P NATL ACAD SCI USA, V98, P4540 BUTLAND G, 2005, NATURE, V433, P531, DOI 10.1038/nature03239 CARTER RA, 2002, MOL PLANT MICROBE IN, V15, P69 COWIE A, 2006, APPL ENVIRON MICROB, V72, P7156, DOI 10.1128/AEM.01397-06 DAVIDSON AL, 2004, ANNU REV BIOCHEM, V73, P241, DOI 10.1146/annurev.biochem.73.011303.073626 DELGADO MJ, 2006, MICROBIOL-SGM 1, V152, P199 DJORDJEVIC MA, 2003, MOL PLANT MICROBE IN, V16, P508 DUPONT L, 2004, J BACTERIOL, V186, P5988 FRY J, 2001, MOL PLANT MICROBE IN, V14, P1016 GAGE DJ, 1998, J BACTERIOL, V180, P5739 GALIBERT F, 2001, SCIENCE, V293, P668 HAMBLIN MJ, 1990, MOL MICROBIOL, V4, P1567 HOSIE AHF, 2001, MOL MICROBIOL, V40, P1449 JEBBAR M, 2005, J BACTERIOL, V187, P1293, DOI 10.1128/JB.187.4.1293-1304.2005 JENSEN JB, 2002, J BACTERIOL, V184, P2978 KANEKO T, 2000, DNA RES, V7, P331 KANEKO T, 2002, DNA RES, V9, P189 KARUNAKARAN R, 2005, MICROBIOL-SGM 10, V151, P3249, DOI 10.1099/mic.0.28311-0 KELLY DJ, 2001, FEMS MICROBIOL REV, V25, P405 KROGAN NJ, 2006, NATURE, V440, P637, DOI 10.1038/nature04670 LAMBERT A, 2001, J BACTERIOL, V183, P4709 MACLELLAN SR, 2006, MICROBIOL-SGM 6, V152, P1751, DOI 10.1099/mic.028743-0 NELSON KE, 1999, NATURE, V399, P323 OVERDUIN P, 1988, MOL MICROBIOL, V2, P767 PAJATSCH M, 1998, J BACTERIOL, V180, P2630 PATZER SI, 1998, MOL MICROBIOL, V28, P1199 PEREGO M, 1991, MOL MICROBIOL, V5, P173 PLATERO RA, 2003, FEMS MICROBIOL LETT, V218, P65 POOLE PS, 1994, MICROBIOL-SGM 10, V140, P2787 REN QH, 2005, PLOS COMPUT BIOL, V1, P190, ARTN e27 RICHARDSON JS, 2004, J BACTERIOL, V186, P8433, DOI 10.1128/JB.186.24.8433-8442.2004 ROSSBACH S, 1994, MOL GEN GENET, V245, P11 SAIER MH, 2000, MICROBIOL MOL BIOL R, V64, P354 SIMON R, 1983, BIO-TECHNOL, V1, P784 STEIN MA, 1997, J BACTERIOL, V179, P6335 STOVER CK, 2000, NATURE, V406, P959 THOMAS GH, 2006, MICROBIOL-SGM 1, V152, P187, DOI 10.1099/MIC.0.28334-0 UCHIUMI T, 2004, J BACTERIOL, V186, P2439, DOI 10.1128/JB.186.8.2439-2448.2004 VANDERPLOEG JR, 1996, J BACTERIOL, V178, P5438 WEBB E, 1998, J BIOL CHEM, V273, P8946 WILLIAMS SG, 1992, MOL MICROBIOL, V13, P1755 WILLIS LB, 1999, J BACTERIOL, V181, P4176 YOUNG JPW, 2006, GENOME BIOL, V7, ARTN R34 YUAN ZC, 2006, NUCLEIC ACIDS RES, V34, P2686, DOI 10.1093/nar/gkl365 Mauchline, T. H. Fowler, J. E. East, A. K. Sartor, A. L. Zaheer, R. Hosie, A. H. F. Poole, P. S. Finan, T. M.

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