Accessibility navigation


A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria

Karunakaran, R., Mauchline, T. H., Hosie, A. H. F. and Poole, P. S. (2005) A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria. Microbiology-Sgm, 151. pp. 3249-3256. ISSN 1350-0872

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1099/mic.0.28311-0

Abstract/Summary

A series of promoter probe vectors for use in Gram-negative bacteria has been made in two broad-host-range vectors, pOT (pBBR replicon) and pJP2 (incP replicon). Reporter fusions can be made to gfpUV, gfprnut3.1, unstable gfpmut3.1 variants (LAA, LVA, AAV and ASV), gfp+, dsRed2, dsRedT3, dsRedT4, mRFP1, gusA or lacZ. The two vector families, pOT and pJP2, are compatible with one another and share the same polylinker for facile interchange of promoter regions. Vectors based on pJP2 have the advantage of being ultra-stable in the environment due to the presence of the parABCDE genes. As a confirmation of their usefulness, the dicarboxylic acid transport system promoter (dctA(p)) was cloned into a pOT (pRU1097)- and a pJP2 (pRU1156)-based vector and shown to be expressed by Rhizobium leguminosarum in infection threads of vetch. This indicates the presence of dicarboxylates at the earliest stages of nodule formation.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10345
Uncontrolled Keywords:GREEN FLUORESCENT PROTEIN, LEGUMINOSARUM BV VICIAE, HOST-RANGE VECTORS, RHIZOBIUM-LEGUMINOSARUM, ROOT-NODULES, VARIANTS, GFP, IDENTIFICATION, REPLICATION, MUTAGENESIS

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation