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Functional and molecular characterization of Tachykinins and tachykinin receptors in the mouse uterus

Patak, E., Pinto, F. M., Story, M. E., Pintado, C. O., Fleming, A., Page, N. M., Pennefather, J. N. and Candenas, M. L. (2005) Functional and molecular characterization of Tachykinins and tachykinin receptors in the mouse uterus. Biology of Reproduction, 72 (5). pp. 1125-1133. ISSN 0006-3363

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To link to this item DOI: 10.1095/biolreprod.104.036814

Abstract/Summary

The aim of this study was to analyze the function and expression of tachykinins, tachykinin receptors, and neprilysin (NEP) in the mouse uterus. A previous study showed that the uterotonic effects of substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) in estrogen-treated mice were mainly mediated by the tachykinin NK, receptor. In the present work, further contractility studies were undertaken to determine the nature of the receptors mediating responses to tachykinins in uteri of late pregnant mice. Endpoint and real-time quantitative RTPCR were used to analyze the expression of the genes that encode the tachykinins SP/NKA, NKB, and hemokinin-1 (HK-1) (Tac1, Tac2, and Tac4); and the genes that encode tachykinin NK1 (Tacr1), NK2 (Tacr2), and NK3 (Tacr3) receptors in uteri from pregnant and nonpregnant mice. The data show that the mRNAs of tachykinins (particularly NKB and HK-1), tachykinin receptors, and NEP are locally expressed in the mouse uterus, and their expression changes during the estrous cycle and during pregnancy. The tachykinin INK, receptor is the predominant tachykinin receptor in the nonpregnant and early pregnant mouse and may mediate tachykinin-induced uterine contractions in the nonpregnant mouse. The tachykinin NK, receptor is predominant in the late pregnant mouse and is the main receptor mediating uterotonic responses to tachykinins at late pregnancy. The tachykinin NK, receptor is expressed in considerable amounts only in uteri from nonpregnant diestrous animals, and its physiological significance remains to be clarified.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10373
Uncontrolled Keywords:female reproductive tract, mouse, neuroendocrinology, pregnancy, signal, transduction, tachykinin NK2, receptor, tachykinins, uterus, ESTROGEN-PRIMED RAT, UTERINE SMOOTH-MUSCLE, NEUROKININ-B, SUBSTANCE-P, PHARMACOLOGICAL PROFILE, MAMMALIAN TACHYKININS, CONTRACTILE RESPONSE, NK2 RECEPTORS, EXPRESSION, NERVES

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