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Improved display of synthetic IgG-binding domains on the baculovirus surface

Ojala, K., Koski, J., Ernst, W., Grabherr, R., Jones, I. and Oker-Blom, C. (2004) Improved display of synthetic IgG-binding domains on the baculovirus surface. Technology in Cancer Research & Treatment, 3 (1). pp. 77-84. ISSN 1533-0346

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Abstract/Summary

Improved display of foreign protein moieties in combination with beneficial alteration of the viral surface properties should be of value for targeted and enhanced gene delivery. Here, we describe a vector based on Autographa californica multiple nucleopolyhedrovirus (AcMNPV) displaying synthetic IgG-bincling domains (ZZ) of protein A fused to the transmembrane anchor of vesicular stomatitis virus (VSV) G protein. This display vector was equipped with a GFP/EGFP expression cassette enabling fluorescent detection in both insect and mammalian cells. The virus construct displayed the biologically active fusion protein efficiently and showed increased binding capacity to IgG. As the display is carried out using a membrane anchor of foreign origin, gp64 is left intact for virus entry, which may increase gene expression in the transduced mammalian cells. In addition, the viral vector can be targeted to any desired cell type via binding of ZZ domains when an appropriate IgG antibody is available.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10568
Uncontrolled Keywords:baculovirus display, ZZ domains, protein A, VSV G protein, targeting, gene delivery, gene therapy, NUCLEAR POLYHEDROSIS-VIRUS, EFFICIENT GENE-TRANSFER, AUTOGRAPHA-CALIFORNICA NPV, GREEN FLUORESCENT PROTEIN, MAMMALIAN-CELLS, RECOMBINANT BACULOVIRUS, IN-VIVO, FOREIGN PROTEINS, G GLYCOPROTEIN, VECTORS

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