Accessibility navigation


Exchangeability of mammalian DNA ligases between base excision repair pathways

Sleeth, K. M., Robson, R. L. and Dianov, G. L. (2004) Exchangeability of mammalian DNA ligases between base excision repair pathways. Biochemistry, 43 (40). pp. 12924-12930. ISSN 0006-2960

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1021/bi0492612

Abstract/Summary

In mammalian cells, DNA ligase IIIalpha and DNA ligase I participate in the short- and long-patch base excision repair pathways, respectively. Using an in vitro repair assay employing DNA ligase-depleted cell extracts and DNA substrates containing a single lesion repaired either through short-patch (regular abasic site) or long-patch (reduced abasic site) base excision repair pathways, we addressed the question whether DNA ligases are specific to each pathway or if they are exchangeable. We find that immunodepletion of DNA ligase I did not affect the short-patch repair pathway but blocked long-patch repair, suggesting that DNA ligase IIIa is not able to substitute DNA ligase I during long-patch repair. In contrast, immunodepletion of DNA ligase IIIa did not significantly affect either pathway. Moreover, repair of normal abasic sites in wild-type and X-ray cross-complementing gene 1 (XRCC1)-DNA ligase IIIalpha-immunodepleted cell extracts involved similar proportions of short- and long-patch repair events. This suggests that DNA ligase I was able to efficiently substitute the XRCC1-DNA ligase IIIa complex during short-patch repair.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10619
Uncontrolled Keywords:STRAND BREAK REPAIR, POLYMERASE-BETA, POLY(ADP-RIBOSE) POLYMERASE, DAMAGING AGENTS, CELL-EXTRACTS, XRCC1, REQUIREMENT, GENE, PROTEINS, RESIDUES

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation