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Improving baculovirus recombination

Zhao, Y., Chapman, D. A. and Jones, I. M. (2003) Improving baculovirus recombination. Nucleic Acids Research, 31 (2). E6-6. ISSN 1362-4962

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Official URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract/Summary

Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection unless rescued by recombination with a baculovirus transfer vector. Modified viral DNA allows 100% recombinant virus formation, obviates the need for further virus purification and offers an efficient means of mass parallel recombinant formation.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:10916
Uncontrolled Keywords:Animals, Bacterial Proteins/genetics, Baculoviridae/*genetics, Cell Line, DNA, Viral/genetics, *DNA-Binding Proteins, Escherichia coli/genetics, *Escherichia coli Proteins, Exodeoxyribonucleases/genetics, Gene Expression, Genes, Viral/genetics, Luminescent Proteins/genetics/metabolism, Mutation, Recombinant Fusion Proteins/genetics/metabolism, *Recombination, Genetic, Support, Non-U.S. Gov't, Transfection/methods

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