Method of plant tissue culture and regeneration
Zeneca Ltd (1999) Method of plant tissue culture and regeneration. US 5,969,215.
Official URL: http://patft.uspto.gov/netacgi/nph-Parser
Plants may be regenerated from stomatal cells or protoplasts of such cells. Prior to regeneration the cells or protoplasts may be genetically transformed by the introduction of hereditary material most preferably by a DNA construct which is free of genes which specify resistance to antibiotics. The regeneration step may include callus formation on a hormone-free medium. The method is particularly suitable for sugar beet.