Transformation of plant cells
Zeneca Ltd (1995) Transformation of plant cells. US 5,464,765.
Official URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PT...
Plant cells are transformed by bringing them into contact with a a multiplicity of needle-like bodies on which the cells may be impaled. This causes a rupture in the cell wall allowing entry of transforming DNA either from a surrounding liquid medium or of DNA previously bound to or otherwise entrapped in the needle-like projections.