Dunwell, J. M., Wilkinson, M. J., Nelson, S., Wening, S., Sitorus, A. C., Mienanti, D., Alfiko, Y., Croxford, A. E., Ford, C. S., Forster, B. P. and Caligari, P. D. S.
Production of haploids and doubled haploids in oil palm.
BMC Plant Biology, 10.
To link to this article DOI: 10.1186/1471-2229-10-218
Background: Oil palm is the world’s most productive oil-food crop despite yielding well below its theoretical
maximum. This maximum could be approached with the introduction of elite F1 varieties. The development of
such elite lines has thus far been prevented by difficulties in generating homozygous parental types for F1
Results: Here we present the first high-throughput screen to identify spontaneously-formed haploid (H) and
doubled haploid (DH) palms. We secured over 1,000 Hs and one DH from genetically diverse material and derived
further DH/mixoploid palms from Hs using colchicine. We demonstrated viability of pollen from H plants and
expect to generate 100% homogeneous F1 seed from intercrosses between DH/mixoploids once they develop
Conclusions: This study has generated genetically diverse H/DH palms from which parental clones can be selected
in sufficient numbers to enable the commercial-scale breeding of F1 varieties. The anticipated step increase in
productivity may help to relieve pressure to extend palm cultivation, and limit further expansion into biodiverse
|Biohybrids International Ltd|
|Date Deposited:||28 Jan 2011 09:17|
|Last Modified:||08 Jun 2015 15:22|
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