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Cryopreservation of winter-dormant apple buds: 1 - variation in recovery with cultivar and winter conditions

Vogiatzi, C., Grout, B.W.W., Wetten, A. and Toldam-Andersen, T.B. (2011) Cryopreservation of winter-dormant apple buds: 1 - variation in recovery with cultivar and winter conditions. CryoLetters, 32 (4). pp. 358-366. ISSN 0143-2044

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Official URL: http://www.cryoletters.org/index.htm

Abstract/Summary

The widely-adopted protocol for the cryopreservation of winter buds of fruit trees, such as Malus and Pyrus, was developed in a region with a continental climate, that provides relatively hard winters with a consequent effect on adaptive plant hardiness. In this study the protocol was evaluated in a typical maritime climate (eastern Denmark) where milder winters can be expected. The survival over two winters was evaluated, looking at variation between seasons and cultivars together with the progressive reduction in survival due to individual steps in the protocol. The study confirms that under such conditions significant variation in survival can be expected and that an extended period of imposed dehydration at -4oC is critical for bud survival. The occurrence of freezing events during this treatment suggests that cryodehydration may be involved, as well as evaporative water loss. To optimize the protocol for maritime environments, further investigation into the water status of the explants during cryopreservation is proposed. Keywords: Malus x domestica, cryopreservation, dormant bud, survival, grafting

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Environmental Biology
ID Code:26044
Uncontrolled Keywords:Malus x domestica, cryopreservation, dormant bud, survival, grafting
Publisher:CryoLetters

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