Accessibility navigation


Cryopreservation of winter-dormant apple: III Bud water status and survival after cooling to -30°c and during recovery from cryopreservation

Downloads

Downloads per month over past year

Vogiatzi, C., Grout, B.W.W. and Wetten, A. (2012) Cryopreservation of winter-dormant apple: III Bud water status and survival after cooling to -30°c and during recovery from cryopreservation. CryoLetters, 33 (2). pp. 160-168. ISSN 0143-2044

[img]
Preview
Text - Published Version
· Please see our End User Agreement before downloading.

368Kb

Official URL: http://www.cryoletters.org/Abstracts/vol_33_2_2012...

Abstract/Summary

Abstract In a continuing study to improve the efficiency of dormant bud cryopreservation for tissues hardened in maritime climates, the water status of dormant buds was monitored between -4°C and recovery from liquid nitrogen (LN). Measurement of water content, simple thermal analysis and differential scanning calorimetry were employed. Buds did not lose water during cooling to, or holding at -30°C indicating that cryodehydration and/or other adaptive responses contributed during this essential step. A bud exotherm that was an artefact of warming was detected due to necessary handling at -4°C before cooling to -30°C. There were no significant differences between cultivars with respect to water status at -30°C or immediately upon rewarming from LN despite significant differences in post-LN survival. Buds rehydrated in 5 days, but up to 14 days may be needed for recovery for some cultivars. In some instances buds could be grafted without rehydration, taking up water across the early graft union.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Environmental Biology
ID Code:28433
Publisher:CryoLetters

Download Statistics for this item.

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation