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Heterozygosity and diversity analysis using mapped single nucelotide polymorphisms in a faba bean inbreeding programme

Cottage, A., Gostkiewicz, K., Thomas, J. E., Borrows, R., Torres, A.-M. and O'Sullivan, D. M. (2012) Heterozygosity and diversity analysis using mapped single nucelotide polymorphisms in a faba bean inbreeding programme. Molecular Breeding, 30 (4). pp. 1799-1809. ISSN 1572-9788

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To link to this item DOI: 10.1007/s11032-012-9745-4

Abstract/Summary

The aim of this study was to convert existing faba bean (Vicia faba L.) single nucleotide polymorphism (SNP) markers from cleaved amplification polymorphic sequences and SNaPshot® formats, which are expensive and time-consuming, to the more convenient KBiosciences competitive allele‐specific PCR (KASP) assay format. Out of 80 assays designed, 75 were validated, though a core set of 67 of the most robust markers is recommended for further use. The 67 best KASP SNP assays were used across two generations of single seed descent to detect unintended outcrossing and to track and quantify loss of heterozygosity, a capability that will significantly increase the efficiency and performance of pure line production and maintenance. This same set of assays was also used to examine genetic relationships between the 67 members of the partly inbred panel, and should prove useful for line identification and diversity studies in the future.

Item Type:Article
Refereed:Yes
Divisions:No Reading authors. Back catalogue items
Interdisciplinary centres and themes > Centre for Food Security
Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:32146
Publisher:Springer

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