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First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain

Unsworth, C.P., Graham, E.S., Delivopoulos, E., Dragunow, M. and Murray, A.F. (2010) First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain. Journal of Neuroscience Methods, 194 (1). pp. 154-157. ISSN 0165-0270

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To link to this item DOI: 10.1016/j.jneumeth.2010.09.022

Abstract/Summary

In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are each key aspects of this new method. The benefits in patterning human neurons on silicon chip using an accessible cell line and robust patterning technology are of widespread value. Thus, using a combined technology such as this will facilitate the detailed study of the pathological human brain at both the single cell and network level.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Department of Bio-Engineering
ID Code:33919
Uncontrolled Keywords:Cell patterning; hNT neuron; NTera2 cell line; NT2; Parylene-C; Human brain cell; Parylene; Silicon chip; On chip
Publisher:Elsevier

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