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First human hNT astrocytes patterned to single cell resolution on parylene-C/Silicon dioxide substrates

Unsworth, C. P., Graham, E.u. S., Delivopoulos, E. and Murray, A. F. (2011) First human hNT astrocytes patterned to single cell resolution on parylene-C/Silicon dioxide substrates. In: 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, pp. 3605-3608. ISBN 978-1-4244-4121-1

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To link to this item DOI: 10.1109/IEMBS.2011.6090604

Abstract/Summary

In our previous work we developed a successful protocol to pattern the human hNT neuron (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/SiO2 substrates. This communication, reports how we have successfully managed to pattern the supportive cell to the neuron, the hNT astrocyte, on such substrates. Here we disseminate the nanofabrication, cell differentiation and cell culturing protocols necessary to successfully pattern the first human hNT astrocytes to single cell resolution on parylene-C/SiO2 substrates. This is performed for varying parylene strip widths providing excellent contrast to the SiO2 substrate and elegant single cell isolation at 10μm strip widths. The breakthrough in patterning human cells on a silicon chip has widespread implications and is valuable as a platform technology as it enables a detailed study of the human brain at the cellular and network level.

Item Type:Book or Report Section
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Department of Bio-Engineering
ID Code:33982
Publisher:IEEE

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