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Evaluation of methods of detecting cell reactive oxygen species production for drug screening and cell cycle studies

Fan, L. M. and Li, J.-M. (2014) Evaluation of methods of detecting cell reactive oxygen species production for drug screening and cell cycle studies. Journal of Pharmacological and Toxicological Methods, 70 (1). pp. 40-47. ISSN 1056-8719

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To link to this item DOI: 10.1016/j.vascn.2014.03.173

Abstract/Summary

Intracellular reactive oxygen species (ROS) production is essential to normal cell function. However, excessive ROS production causes oxidative damage and cell death. Many pharmacological compounds exert their effects on cell cycle progression by changing intracellular redox state and in many cases cause oxidative damage leading to drug cytotoxicity. Appropriate measurement of intracellular ROS levels during cell cycle progression is therefore crucial in understanding redox-regulation of cell function and drug toxicity and for the development of new drugs. However, due to the extremely short half-life of ROS, measuring the changes in intracellular ROS levels during a particular phase of cell cycle for drug intervention can be challenging. In this article, we have provided updated information on the rationale, the applications, the advantages and limitations of common methods for screening drug effects on intracellular ROS production linked to cell cycle study. Our aim is to facilitate biomedical scientists and researchers in the pharmaceutical industry in choosing or developing specific experimental regimens to suit their research needs.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:46383
Publisher:Elsevier

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