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Detection of the phosphorylation of the estrogen receptor alpha as an outcome of GPR30 activation

Clark, S., Pollard, K., Rainville, J. and Vasudevan, N. (2015) Detection of the phosphorylation of the estrogen receptor alpha as an outcome of GPR30 activation. In: Eyster, K. M. (ed.) Estrogen receptors: methods and protocols. Methods in Molecular Biology, 1366. Humana Press, New York, pp. 457-470. ISBN 9781493931262

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To link to this item DOI: 10.1007/978-1-4939-3127-9_36

Abstract/Summary

Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modifi cation events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.

Item Type:Book or Report Section
Refereed:Yes
Divisions:No Reading authors. Back catalogue items
ID Code:58940
Publisher:Humana Press

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