Accessibility navigation


Microsatellite markers for hoop-petticoat daffodils (Narcissus sect. Bulbocodii; Amaryllidaceae)

Konyves, K., David, J. C. and Culham, A. (2016) Microsatellite markers for hoop-petticoat daffodils (Narcissus sect. Bulbocodii; Amaryllidaceae). Applications in Plant Sciences, 4 (4). 1500127. ISSN 2168-0450

[img]
Preview
Text (Open Access) - Published Version
· Available under License Creative Commons Attribution Non-commercial Share Alike.
· Please see our End User Agreement before downloading.

547kB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.3732/apps.1500127

Abstract/Summary

• Premise of the study: Microsatellite markers were developed using hoop-petticoat daffodils ( Narcissus sect. Bulbocodii ; Amaryllidaceae) to aid in the taxonomic revision of the section, and further to evaluate their broad applicability for daffodil cultivar identification. • Methods and Results: Three hundred fifty-one primer pairs were developed using a commercial service. Nineteen polymorphic and repeatable markers were developed by screening 67 of these primer pairs. Of these, 11 chosen markers were used to screen 317 samples; the number of alleles per locus ranged from four to 21, and the observed heterozygosity ranged from 0.101 to 0.297. There were null genotypes in some samples for six of the markers. All the microsatellites were transferable to other Narcissus sections. • Conclusions: The results indicate that these new markers have sufficient potential variation to be used for taxonomic revision of the genus and to distinguish many commercial daffodil cultivars.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Ecology and Evolutionary Biology
Central Services > Academic and Governance Services > University Museums and Special Collections
ID Code:62397
Uncontrolled Keywords:microsatellite, taxonomy, DNA marker, Daffodil, Narcissus, Amaryllidaceae, daffodil cultivars, horticultural taxonomy; microsatellite markers; Narcissus section Bulbocodii, polyploidy
Additional Information:Funded by the Royal Horticultural Society
Publisher:Botanical Society of America

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation