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Cryopreservation of winter-dormant apple buds IV: critical temperature variation that can compromise survival

Vogiatzi, C., Grout, B. W. W., Wetten, A., Ordidge, M. and Clausen, S. K. (2018) Cryopreservation of winter-dormant apple buds IV: critical temperature variation that can compromise survival. CryoLetters, 39 (4). pp. 245-250. ISSN 0143-2044

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Official URL: http://www.cryoletters.org/Abstracts/vol_39_4_2018...

Abstract/Summary

BACKGROUND: Precise temperature control in several key areas during cryopreservation of dormant, winter apple buds is critical for maximal survival. OBJECTIVE: To consider the effects of pre-harvest temperature, the duration of incubation at -30°C and variation in rewarming rate on survival. MATERIALS AND METHODS: Dormant winter buds of Malus x domestica cultivars were harvested with two different acclimation histories and cryopreserved with variation in incubation time at -30°C. Recovery from LN using slow, intermediate and rapid rewarming was investigated as well as preservation after prolonged storage at -4°C. RESULTS: The effects on survival of preharvest temperature regime and an altered -30°C incubation regime are cultivar dependent and an increase in rewarming rate has a strong negative effect on recovery. CONCLUSION: Post-thaw survival of the winter-dormant buds can be compromised by increased temperature over a period as short as 5 days prior to bud harvest. Varying incubation times at -30°C produce variable, cultivar dependent, survival and moderate increases in rewarming rates can also radically reduce survival.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:79787
Publisher:CryoLetters

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