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In vitro genetics of barley (Hordeum vulgare L.): response of immature embryos to 2,4-dichlorophenoxyacetic acid

Powell, W. and Dunwell, J. M. (1987) In vitro genetics of barley (Hordeum vulgare L.): response of immature embryos to 2,4-dichlorophenoxyacetic acid. Heredity, 58. pp. 75-80. ISSN 1365-2540

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To link to this item DOI: 10.1038/hdy.1987.11

Abstract/Summary

This study involved the response of immature embryos of nine cultivars of spring barley to culture on media containing 2,4-dichiorophenoxyacetic acid (2,4-D). Each embryo, with any attached callus, was harvested after 28 days culture and its shoot length, root length, fresh weight, dry weight and water content was recorded. For each character, there were significant differences between genotypes, between 2,4-D concentrations and also significant genotype x medium interactions. These interactions were successfully analysed using either a joint regression analysis, or a genotype's phenotypic variance over the range of 2,4-D concentrations. This two dimensional assessment of the response of a genotype to culture environment may be useful in defining more critically the parameters involved in the control of barley cell proliferation in vitro.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:86953
Publisher:Nature Publishing Group

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