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Effect of anther orientation on microspore-callus production in barley (Hordeum vulgate L.)

Shannon, P. R. M., Nicholson, A. E., Dunwell, J. M. and Davies, D. R. (1985) Effect of anther orientation on microspore-callus production in barley (Hordeum vulgate L.). Plant Cell, Tissue and Organ Culture, 4 (3). pp. 271-280. ISSN 1573-5044

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To link to this item DOI: 10.1007/BF00040201


Barley anthers from cold pretreated spikes produced no or few calluses when lated with both loculi in contact with the medium ('flat'). When anthers were plated with only one loculus in contact with the medium ('up'), a high proportion of the anthers produced calluses. The top loculus of the 'up' anthers was most productive. 'Flat' anthers, when compared with 'up' anthers, were not only slower to produce multicellular pollen grains (MCPs) and microcalluses, but also produced fewer of them and ceased production earlier. The MCPs and microcalluses in 'flat' anthers grew more slowly and few developed beyond the 30 cell stage. These results establish the importance of anther orientation for barley anther culture.

Item Type:Article
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:86967
Uncontrolled Keywords:androgenesis, anther culture, barley, haploid, Hordeum vulgare L., pollen

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