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Anther culture of Sorghum bicolor (L.) Moench I. Effect of panicle pretreatment, anther incubation temperature and 2,4-D concentration

Rose, J. B., Dunwell, J. M. and Sunderland, N. (1986) Anther culture of Sorghum bicolor (L.) Moench I. Effect of panicle pretreatment, anther incubation temperature and 2,4-D concentration. Plant Cell, Tissue and Organ Culture, 6 (1). pp. 15-22. ISSN 1573-5044

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To link to this item DOI: 10.1007/BF00037754

Abstract/Summary

In a study of anther culture in Sorghum bicolor it was found that culture incubation temperature had a significant effect on yields of microspore-derived callus. A temperature of 33°C was superior to 25 °, 28 ° or 37 ° C. With fresh, unpretreated material, callus was produced only from anthers incubated at 33 ° C. Panicle pretreatment at 7 ° , 14 ° , 20 ° , 25 ° or 28 ° C either had no effect or signifi- cantly decreased callus yield. Comparison of culture media containing a range of concentrations of 2,4-dichloro- phenoxyacetic acid (0-3.0 mg 1-1) showed that callus yield increased significantly with concentration up to 2.5 mg1-1 and then declined slightly. Although 1174 calluses were produced, subsequent differentiation was poor and only four albino plants were regenerated.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:86970
Uncontrolled Keywords:Sorghum bicolor, anther culture, haploid, pollen
Publisher:Springer

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