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Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos

Roberts-Oehlschlager, S. L. and Dunwell, J. M. (1990) Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos. Plant Cell, Tissue and Organ Culture, 20 (3). pp. 235-240. ISSN 0167-6857

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Official URL: https://link.springer.com/article/10.1007/BF000418...

Abstract/Summary

A period of four days preincubation at 25 °C on a medium containing mannitol was found to be superior to those pretreatments requiring incubation at 4 °C. In addition, the yield of green plants was improved by orienting anthers 'flat' on the medium during mannitol preincubation, and reducing the number of anthers cultured per dish.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:86986
Uncontrolled Keywords:anther, barley, mannitol, microspores, pretreatments
Publisher:Springer

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