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Cadmium isotope fractionation reveals genetic variation in Cd uptake and translocation by Theobroma cacao and role of Natural Resistance-Associated Macrophage Protein (NRAMP5) and Heavy Metal ATPases (HMA)-family transporters

Moore, R. E. T., Ullah, I., de Oliveira, V. H., Hammond, S. J., Strekopytov, S., Tibbett, M., Dunwell, J. and Rehkamper, M. (2020) Cadmium isotope fractionation reveals genetic variation in Cd uptake and translocation by Theobroma cacao and role of Natural Resistance-Associated Macrophage Protein (NRAMP5) and Heavy Metal ATPases (HMA)-family transporters. Horticulture Research, 7. 71. ISSN 2052-7276

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To link to this item DOI: 10.1038/s41438-020-0292-6

Abstract/Summary

In response to new EU regulations, studies are underway to mitigate accumulation of toxic cadmium (Cd) in cacao (Theobroma cacao, Tc). This study advances such research with Cd isotope analyses of 19 genetically diverse cacao clones and yeast transformed to express cacao Natural Resistance-Associated Macrophage Protein NRAMP5 and Heavy Metal ATPases (HMAs). The plants were enriched in light Cd isotopes relative to the hydroponic solution with Δ114/110Cdtot-sol= –0.22 ± 0.08‰. Leaves show a systematic enrichment of isotopically heavy Cd relative to total plants, in accord with closed-system isotope fractionation of Δ114/110Cdseq-mob= –0.13‰, by sequestering isotopically light Cd in roots/stems and mobilisation of remaining Cd to leaves. The findings demonstrate that (i) transfer of Cd between roots and leaves is primarily unidirectional; (ii) different clones utilise similar pathways for Cd sequestration, which differ from those of other studied plants; (iii) clones differ in their efficiency of Cd sequestration. Transgenic yeast that expresses TcNRAMP5 had isotopically lighter Cd than did cacao. This suggests that NRAMP5 transporters constitute an important pathway for uptake of Cd by cacao. Cadmium isotope signatures of transgenic yeast expressing HMA-family proteins suggest they may contribute to Cd sequestration. The data are the first to record isotope fractionation induced by transporter proteins in vivo.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:89638
Publisher:Springer Nature

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