Accessibility navigation


Intact myocardial preparations reveal intrinsic transmural heterogeneity in cardiac mechanics

Pitoulis, F. G., Hasan, W., Papadaki, M., Clavere, N. G., Perbellini, F., Harding, S. E., Kirk, J. A., Boateng, S., de Tombe, P. P. and Terracciano, C. M. (2020) Intact myocardial preparations reveal intrinsic transmural heterogeneity in cardiac mechanics. Journal of Molecular and Cellular Cardiology, 141. pp. 11-16. ISSN 0022-2828

[img] Text - Accepted Version
· Restricted to Repository staff only until 19 March 2021.
· Available under License Creative Commons Attribution Non-commercial No Derivatives.

1MB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1016/j.yjmcc.2020.03.007

Abstract/Summary

Determining transmural mechanical properties in the heart provides a foundation to understand physiological and pathophysiological cardiac mechanics. Although work on mechanical characterisation has begun in isolated cells and permeabilised samples, the mechanical profile of living individual cardiac layers has not been examined. Myocardial slices are 300 μm-thin sections of heart tissue with preserved cellular stoichiometry, extracellular matrix, and structural architecture. This allows for cardiac mechanics assays in the context of an intact in vitro organotypic preparation. In slices obtained from the subendocardium, midmyocardium and subepicardium of rats, a distinct pattern in transmural contractility is found that is different from that observed in other models. Slices from the epicardium and midmyocardium had a higher active tension and passive tension than the endocardium upon stretch. Differences in total myocyte area coverage, and aspect ratio between layers underlined the functional readouts, while no differences were found in total sarcomeric protein and phosphoprotein between layers. Such intrinsic heterogeneity may orchestrate the normal pumping of the heart in the presence of transmural strain and sarcomere length gradients in the in vivo heart.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:89907
Publisher:Elsevier

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation