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2-oxoglutarate-dependent dioxygenases: a renaissance in attention for ascorbic acid in plants

Mahmood, A. M. and Dunwell, J. M. ORCID: https://orcid.org/0000-0002-6281-9797 (2020) 2-oxoglutarate-dependent dioxygenases: a renaissance in attention for ascorbic acid in plants. PLoS ONE, 15 (12). e0242833. ISSN 1932-6203

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To link to this item DOI: 10.1371/journal.pone.0242833

Abstract/Summary

-Ascorbicacid(ascorbate,VitaminC)is an essential human micronutrient that is predominantly obtained from plants.It is known to work as the major antioxidant in plants,and it underpins several environmentally induced stresses dueto its use as a co-factor by certain 2-oxoglutarate-dependent(2-OG)dioxygenases[2(OG)-dioxygenases].It is important to understand the role of 2(OG)-dioxygenases in the biosynthesis of ascorbate.The present study examined contents of ascorbate andprotein-protein interaction in nine T-DNA mutants of Arabidopsis containing an insert in their respective(2-OG)dioxygenase genes(At1g20270, At1g68080, At2g17720, At3g06290, At3g28490, At4g35810, At4g35820, At5g18900, At5g66060)In this study,the amount of ascorbate in five of the mutants was shown to be almost two-fold or more than two-fold higher than in the wildtype.This result may be a consequence of the insertion of theT-DNA. The prediction of possible protein interactions between2(OG)-dioxygenases and relevant ascorbate-function players may indicate the oxidative effects of certain dioxygenase proteins in plants.It is expected that certain dioxygenases areactively involvedin the metabolic and biosynthetic pathways of ascorbate.This involvement may be of importance to increase ascorbate amounts in plants for human nutrition,and to protect plant species against stress conditions.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:94829
Publisher:Public Library of Science

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