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Sterol content analysis suggests altered eburicol 14 alpha-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides

Bean, T. P., Cools, H. J., Lucas, J. A., Hawkins, N. D., Ward, J. L., Shaw, M. W. and Fraaije, B. A. (2009) Sterol content analysis suggests altered eburicol 14 alpha-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides. FEMS Microbiology Letters, 296 (2). pp. 266-273. ISSN 0378-1097

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To link to this article DOI: 10.1111/j.1574-6968.2009.01645.x

Abstract/Summary

The recent decline in the effectiveness of some azole fungicides in controlling the wheat pathogen Mycosphaerella graminicola has been associated with mutations in the CYP51 gene encoding the azole target, the eburicol 14 alpha-demethylase (CYP51), an essential enzyme of the ergosterol biosynthesis pathway. In this study, analysis of the sterol content of M. graminicola isolates carrying different variants of the CYP51 gene has revealed quantitative differences in sterol intermediates, particularly the CYP51 substrate eburicol. Together with CYP51 gene expression studies, these data suggest that mutations in the CYP51 gene impact on the activity of the CYP51 protein.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Centre for Food Security
Faculty of Life Sciences > School of Biological Sciences
ID Code:9550
Uncontrolled Keywords:Septoria tritici, azole fungicide, eburicol 14 alpha-demethylase, CYP51, sterol content, RESISTANT CANDIDA-ALBICANS, ANTIFUNGAL AGENTS, AIDS PATIENTS, FLUCONAZOLE, MECHANISMS, AMPHOTERICIN, MUTATIONS, STRAINS

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