Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization
Hardesty, B. D., Hughes, S. L., Rodriguez, V. M. and Hawkins, J. A. (2008) Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization. Molecular Ecology Resources, 8 (1). pp. 164-167. ISSN 1471-8278
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To link to this article DOI: 10.1111/j.1471-8286.2007.01913.x
Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.
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